The type of method that was used for analyzing the
sample was the tube method with the tile method of ABO cell grouping
sample was the tube method with the tile method of ABO cell grouping
Principle
ABO blood
grouping anti-sera react with unknown red cell antigen form agglutination
(Abayomi, 2007).
grouping anti-sera react with unknown red cell antigen form agglutination
(Abayomi, 2007).
Procedure for Tube Method
i.
A 2-5% cell suspension of the control and test
cells was prepared.
ii.
Glass tubes were arranged on the rack in three
rows.
iii.
A volume of anti-A and saline was placed in each
tube in the first row.
iv.
A volume of anti- B and saline was placed in
each of the tube in the second row.
v.
A volume of anti-A + anti- B and saline were
placed in each of the tube in the third row.
vi.
An auto-agglutin served as control and a volume
of each cell suspension was put against its own serum.
vii.
One volume of the control cells suspension was
placed into the first rows and process repeated for the B, O and test cells.
viii.
The tubes were tapped to ensure that the cells
and serum were thoroughly mixed and then allow to stand in room temperature for
1 ½.
The tubes were tapped to ensure that the cells
and serum were thoroughly mixed and then allow to stand in room temperature for
1 ½.
ix.
Each tube was removed, tapped to check for
agglutination each tubes were removed starting with the auto agglutin control,
agitated by tapping and check for agglutination .
Procedure for tile Method
i.
A white tile was cleaned with ordinary cotton
wool and placed on a work bench.
ii.
A drop of blood was placed in four (4) rows.
iii.
A drop of equal volume of commercial anti-sera was
added to the whole blood as follows:
iv.
First row anti-sera A, second row anti-sera B,
third row anti-sera AB, fourth row anti-sera O.
v.
The blood and anti-sera were mixed together
using separate applicator stick for each row.
vi.
The tube was rocked gently to check for
agglutination macroscopically.
